Thin Layer Chromatography

Thin-Layer Chromatography (TLC) is a separation technique for both qualitative and quantitative analysis, enabling simultaneous analysis of many substances with minimal time requirement. High-Performance Thin-Layer Chromatography (HPTLC) is the most advanced form of TLC. As opposed to other separation techniques, the initial costs for an HPTLC system as well as maintenance, and costs per sample are comparatively low, and the possibility of visual evaluation of separated samples on the plate is one of the most valuable aspects of TLC.

Sample application is the first step in the workflow of planar chromatography. Samples are applied onto the plate either as spots or as bands. The precision of the applied volume, the exact positioning and the compactness of the application zone determine the quality of the final result.

Three different models are available:
Nanomat 4 and Capillary Dispenser for easy application of samples in the form of spots onto TLC/HPTLC layers.
Linomat 5 for samples sprayed automatically onto TLC/HPTLC plates in the form of bands.
Automatic TLC Sampler 4 (ATS 4) for fully automatic sample application for qualitative and quantitative analyses as well as for preparative separations. More info

Thin-layer chromatography differs from all other chromatographic techniques in the fact that in addition to stationary and mobile phases, a gas phase is present. This gas phase can significantly influence the result of the separation. Samples are separated into their components which remain in their position on the layer after the mobile phase has been evaporated.

Following instruments are available:
Glass chambers for manual separations.
ADC 2 (Automatic Development Chamber) for automated isocratic applications.
AMD 2 (Automatic Multiple Development) for gradient separations. More info

Substances without chromophores or color can be visualized and made detectable through derivatization. The required reagents are applied to the chromatogram by spraying or immersion.

Derivatization devices include:
Chromatogram Immersion Device
TLC/HPTLC Sprayers
TLC Spray Cabinet
TLC Plate Heater More info

The new CAMAG Derivatizer sets the benchmark in the field of automated derivatization of TLC plates. With its state of the art “micro droplet” spraying technology, Camag achieves a standard deviation to a limit of 4,5% and thus outperforms by far the manual spraying method. Additionally, the instrument delivers user-independent and reproducible results notwithstanding the reagent. To meet the diverging physicochemical properties of the reagents, the device is equipped with four different color-coded spray nozzles. In contrast to the dipping variant of TLC plates, requires the spraying process of the Derivatizer much less reagent (2-4 ml per plate) making it better off in terms of cost efficiency. More info

The CAMAG TLC Scanner 4 is the most advanced workstation for densitometric evaluation of TLC/HPTLC chromatograms and other planar objects.

The chromatogram is evaluated under white or UV light. Options range from visual inspection of electronic images to quantitative determinations using video or scanning densitometry. A laboratory fully equipped for instrumental Thin-Layer Chromatography should be able to resort to both classical densitometry and electronic image acquisition. More info

Camag’s TLC Visualizer documentation system captures images of TLC or HPTLC plates. The system provides illumination with white light (remission, transmission or a combination of both), UV 254 nm and UV 366 nm.

Camag’s BioLuminizer is a detection system specifically designed to detect bioluminescence on HPTLC plates.

Camag’s TLC-MS Interface 2 is a highly convenient and versatile instrument allowing for rapid and contamination-free elution of TLC/HPTLC zones with online transfer to a mass spectrometer. More info