Thin Layer Chromatography (TLC/HPTLC)

High-performance thin-layer chromatography (HPTLC) is an enhanced form of thin-layer chromatography (TLC). The high-performance thin-layer chromatography (HPTLC) workflow comprises of the steps sample application, chromatogram development, derivatization and detection. In the sample application step, extracts are applied as bands onto the plate with a software-controlled applicator (Nanomat 4, Linomat 5, ATS 4). For the chromatogram development, the use of automatic development chambers (ADC 2, AMD 2) allow making this step more reproducible and less dependent on human interaction. In the derivatization step, substances without chromophores or fluorophores can be visualized or made detectable through derivatization (CAMAG Derivatizer). In the detection step, a digital camera captures the chromatogram by means of electronic images, which can be further analyzed using the TLC Visualizer 2 or BioLuminizer. Alternatively, chromatograms can be densitometrically evaluated using the TLC Scanner 4. TLC-MS coupling allows for verification of the chemical structure of analytes by mass spectrometry using the CAMAG MS-Interface (TLC-MS Interface 2).
Dried blood spot (DBS) analysis method shows strong advantages compared to the conventional collection and analysis of blood or plasma samples. The fully automated CAMAG DBS-MS 500 is used for the analysis of target substances out of a DBS.

Sample application is the first step in the workflow of planar chromatography. Samples are applied onto the plate either as spots or as bands. The precision of the applied volume, the exact positioning and the compactness of the application zone determine the quality of the final result.

Three different models are available:
Nanomat 4 and Capillary Dispenser for easy application of samples in the form of spots onto TLC/HPTLC layers.
Linomat 5 for samples sprayed automatically onto TLC/HPTLC plates in the form of bands.
Automatic TLC Sampler 4 (ATS 4) for fully automatic sample application for qualitative and quantitative analyses as well as for preparative separations. More information

Thin-layer chromatography differs from all other chromatographic techniques in the fact that in addition to stationary and mobile phases, a gas phase is present. This gas phase can significantly influence the result of the separation. Samples are separated into their components which remain in their position on the layer after the mobile phase has been evaporated.

Following instruments are available:
Glass chambers for manual separations.
ADC 2 (Automatic Development Chamber) for automated isocratic applications.
AMD 2 (Automatic Multiple Development) for gradient separations. More information

Substances without chromophores or color can be visualized and made detectable through derivatization. The required reagents are applied to the chromatogram by spraying or immersion.

Derivatization devices include:
Chromatogram Immersion Device
TLC/HPTLC Sprayers
TLC Spray Cabinet
TLC Plate Heater More information

The new CAMAG Derivatizer sets the benchmark in the field of automated derivatization of TLC plates. With its state of the art “micro droplet” spraying technology, Camag achieves a standard deviation to a limit of 4,5% and thus outperforms by far the manual spraying method. Additionally, the instrument delivers user-independent and reproducible results notwithstanding the reagent. To meet the diverging physicochemical properties of the reagents, the device is equipped with four different color-coded spray nozzles. In contrast to the dipping variant of TLC plates, requires the spraying process of the Derivatizer much less reagent (2-4 ml per plate) making it better off in terms of cost efficiency. More information

The CAMAG TLC Scanner 4 (densitometer) is the most advanced workstation for densitometric evaluation of TLC/HPTLC chromatograms and other planar objects.

The chromatogram is evaluated under white or UV light. The densitometer offers options from visual inspection of electronic images to quantitative determinations using video or scanning densitometry. A laboratory fully equipped for instrumental Thin-Layer Chromatography should be able to resort to both classical densitometry and electronic image acquisition. More information

Camag’s TLC Visualizer 2 is a professional imaging and documentation system for TLC/HPTLC chromatograms and other planar objects with a state-of-the-art digital CCD camera, with which a maximum resolution of 82 μm on the plate is obtained. The system provides illumination with white light (remission, transmission or a combination of both), UV 254 nm and UV 366 nm.

Camag’s BioLuminizer is a detection system specifically designed to detect bioluminescence on HPTLC plates. The system consists of a compartment excluding any extraneous light, climate controlled for extended stability of the plate, and a 16-bit CCD digital camera of high resolution and high quantum efficiency. More information

Camag’s TLC-MS Interface 2 is a highly convenient and versatile instrument allowing for rapid and contamination-free elution of TLC/HPTLC zones with online transfer to a mass spectrometer.

The Interface 2 can be installed plug & play with any LC-MS system without adjustments or mass spectrometer modifications. Known substances can be identified within a minute via their mass spectrum and for an unknown substance zone, the respective sum formula can be obtained.

The Interface 2 offers a highly-effective backwashing function that prevents the elution path from becoming blocked and the easy handling ensures accurate and reproducible plate positioning. More information

HPTLC Pro Module Application is part of the CAMAG® HPTLC PRO SYSTEM, the first fully automated HPTLC system.

HPTLC Pro Module Application is a fully automated sample analysis and evaluation system using HPTLC plates (20 x 10 cm). The HPTLC PRO SYSTEM (patented technology) is best suited for routine quality control of analytes extracted from complex matrices, providing reproducible and reliable results.

It supports up to 75 samples, up to five plates, and up to three independent developing solvents without intervention. The possibility of derivatization, MS coupling, and plate storage are included.

Users have the choice to operate a module as stand-alone or as part of the HPTLC PRO SYSTEM. More information

The usage of dried blood spots (DBS) has strong advantages over conventional collection and analysis of blood or plasma samples, such as lower blood volumes, easier collection of blood samples and easy transportation of samples. The fully automated CAMAG DBS-MS 500 extraction system makes DBS analysis remarkably easy, fast, efficient and economical compared to both the manual and semi-automated punching techniques. Fields of applications include newborn screening (NBS), where only a very small amount of blood is available and therapeutic drug monitoring (TDM). More information